Gene Expression Profiling and Copy Number Variation
We offer low-, medium- and high-throughput options for gene expression and gene copy number analysis of human and mouse samples. Array-comparative genomic hybridization (Array CGH) enables high-resolution detection of genomic copy number variants. Differentially labeled reference and experimental samples are hybridized to microarrays, allowing detection of structural variants as small as 200bp. We offer pre-validated assay panels as well as the capacity to design and validate custom panels. Our platforms include both traditional qPCR-based detection and high-throughput microfluidic chips and microarrays, all with fully automated processing.
Low to Medium Throughput Gene Expression Services
Solutions for tens to hundreds of samples and tens to hundreds of target genes.
Capabilities
- Automated DNA and RNA extraction for a variety of primary sample types (tissue, whole blood, FFPE, cells)
- Integrated sample and data QC
- Automated liquid-handling for PCR reaction setup
- Variety of chemistries available: Taqman®, SYBR Green, Universal ProbeLibrary
- Assay design and wet bench validation at no additional cost
- Differential expression analysis and reports provided
Technologies
- Applied Biosystems ViiA™ 7 Real-Time PCR System- This high performance instrument delivers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. This system combines 96- and 384-well formats with fully automated robotic plate loading for high-productivity real-time PCR processing.
- Fluidigm BioMarkTM System using 96.96 and 48.48 Dynamic Arrays – capable of 9,200 real-time qPCR reactions in picoliter volumes in a single run. Digital PCR arrays enable ultrahigh-resolution and absolute molecule quantification for gene copy number experiments
- Applied Biosystems QuantStudio 5 Real-Time PCR System, 384-well
High Throughput Gene Expression Services
Microarray solutions for transcriptomics and genome-wide expression analysis.
Capabilities
- Automated DNA and RNA extraction for a variety of primary sample types (tissue, whole blood, FFPE, cells)
- Integrated sample and data QC
- Whole-transcriptome amplification (WTA) services for limited amounts of precious RNA samples
- Automated microarray sample processing and array development
- Bioinformatics services for in depth microarray analysis, visualization and interpretation of results
Technologies
IBX offers high throughput microarray-based solutions for gene profiling studies. All sample preparation protocols are fully automated to ensure contaminant-free, robust gene expression analysis across platforms. Integrated sample management, nucleic acid extraction, microarray and analysis service bundles are available.
- Affymetrix GeneChip and GeneTitan Systems for all Affymetrix gene profiling products
- Illumina iScan System for all Illumina microarray products
